Figure 1
Schematic outline of the resequencing strategy using chip-based mass spectrometry. (A) The target region is first PCR-amplified with primers bearing bacteriophage T7 and SP6 RNA polymerase promoter sequences (dashed lines).(B) A mosaic transcript, with dCMP or dTMP/dUMP replacing the regular nucleoside, is derived from each strand of the amplicon (represented by the arrows) and base-specifically cleaved (see text for details).(C) Finally, the set of cleavage products, as a group, is analyzed by an array mass spectrometer.
