Thermostability and double D-loop yield. The gel compares the incubation temperature effects on the yield of joint molecule formation. Sets of four reactions were incubated at each of the temperatures indicated above. In the first reaction of each temperature set, Cy5-labeled incoming oligonucleotides are used alone to show the need for the annealing oligonucleotide. In the second reaction of each set, Cy5-labeled incoming is used with an unlabeled 15-mer LNA annealing oligonucleotide. In the third reaction of each set, unlabeled incoming is used with a Cy5-labeled LNA annealing oligonucleotide. And in the fourth reaction of each set, the Cy5-labeled annealing is used without an incoming oligonucleotide to show the need for the RecA filament. The arrow indicates the position of the double D-loop complex as detected by fluorescence.
