A New Method for Rapidly Generating Gene-Targeting Vectors by Engineering BACs Through Homologous Recombination in Bacteria

(Downloading may take up to 30 seconds. If the slide opens in your browser, select File -> Save As to save it.)

Click on image to view larger version.

Figure 2
Figure 2

“Knock-in” vector construction. (a–d) Sequential steps leading to a mutated NOD2 exon 10 “knock-in” BAC and targeting construct (see text for details); (e) Gel electrophoresis depicting PCR products confirming the recombinants schematized in diagrams: (c) lane 1 (WT-BAC), (d) lane 2 (BACloxP/exon 10m/loxP/aph/loxP), and (e) lane 3 (the targeting vector), generated with P5 and P6.

This Article

  1. Published in Advance January 1, 2003, doi: 10.1101/gr.1356503 Genome Res. 13: 2190-2194

Preprint Server



Current Issue

  1. January 2026, 36 (1)
  1. Current Issue
  1. Alert me to new issues of Genome Research