A New Method for Rapidly Generating Gene-Targeting Vectors by Engineering BACs Through Homologous Recombination in Bacteria

(Downloading may take up to 30 seconds. If the slide opens in your browser, select File -> Save As to save it.)

Click on image to view larger version.

Figure 1
Figure 1

Conditional knockout vector construction. (a–f) Sequential steps leading to a NOD2 exon 3 recombinant BAC and targeting vector (see text for details). (g) Gel electrophoresis depicting PCR products confirming the recombinants schematized in diagrams: (a) lane 1 (WT-BAC), (b) lane 2 (BACloxP/frt/aph/frt), and (c) lane 3 (BACloxP), generated with primers 1 (P1) and 2 (P2); (d) lane 4 (BACloxP) and (e) lane 5 (BACloxP-loxP/aph/loxP), generated with P3 and P4; and (f) lanes 6,7 (targeting construct) using respectively P3 and P4, and P1 and P4. Circled “F”, frt site; large arrowhead, loxP site.

This Article

  1. Published in Advance January 1, 2003, doi: 10.1101/gr.1356503 Genome Res. 13: 2190-2194

Preprint Server



Current Issue

  1. January 2026, 36 (1)
  1. Current Issue
  1. Alert me to new issues of Genome Research