Evaluation of the diagnostic test for variation in gene copy number. (A) Application of the diagnostic test for quantification of KIT copy numbers in a wild boar/Large White intercross (founders, n = 10; F1, n = 22; F2, n = 177). The results obtained for the 1st and 2nd replicate analysis of each sample are presented. The plotted value represents the C/(C+G) ratio, i.e., C being specific for the duplicated copy and G specific for the nonduplicated copy of the gene. The symbol used for each sample represents the copy number predicted on the basis of previous results. (B) Comparison of the estimation of KIT copy numbers for 108 pigs from white commercial lines using pyrosequencing technology and the Invader assay. The results given for the Invader assay are the ratio obtained by dividing the net KIT signal (minus background) by the net ESR signal (minus background).
