(A) Design of a DNA test for quantification of the gene copy number at the porcine KIT locus. The normal copy was PCR-amplified using the shared forward primer (KITBPF) and the specific reversed primer KIT1BPR, or alternatively a mix (1:100) of the two reversed primers KIT1BPtailR and TailR. Similarly, the duplication breakpoint was amplified using KITBPF and KIT2BPR or a mix (1:100) of KIT2BPtailR and TailR. The primer KITBPseq was used for pyrosequencing. (B) Pyrogram obtained when mixing BAC clone 1041B3 containing a nonduplicated KIT copy and increasing proportions of BAC clone 953F11 including the duplication breakpoint. The C and G nucleotides used for quantification are boxed.
