
(A) S1 and S2 are sequenced contigs, and C1, C2, and C3 are clones. The underlined sequence represents the BESs and where they hit on the seqCtg. Note, all lengths of sequences are tremendously reduced to fit on the page. (B) There are four possible pairs, shown in the four rows in the table. A + indicates the BES was not reverse complemented to match the seqCtg. An rc indicates that it was reverse complemented. The seqCtg-BES hits for the first two pairs both have the same orientations, whereas the orientations for the next two are different. The first two are rejected as candidate MTP pairs, wheras the second two are retained.











