Observed Differences Between Two Copies of the ALS7 Locus in the Same Strain and Differences Expected from Random Mixing of Alleles
|
|
Differencesa observedb; average ± SD (n) |
Differencesa expectedc average ± SD (n) |
Significance (P)d |
|---|---|---|---|
| Tandem repeat domain | |||
| All | 2.0 ± 3.1 (66) | 6.4 ± 5.4 (17424) | 8 × 10-11 |
| GPG cluster strains | 1.5 ± 1.9 (42) | 4.4 ± 4.9 (7056) | 0.0001 |
| Noncluster strains | 2.8 ± 4.3 (24) | 7.2 ± 5.7 (2304) | 0.0002 |
| VASES region | |||
| All | 282 ± 352 (66) | 447 ± 464 (17424) | 7 × 10-15 |
| GPG cluster strains | 231 ± 297 (42) | 263 ± 394 (7056) | 0.5915 |
| Noncluster strains
|
373 ± 424 (24)
|
553 ± 466 (2304)
|
0.0588
|
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↵a Differences are expressed as differences in the numbers of repeats for the tandem repeat domain and as differences in the sizes (bp) of the VASES domain.
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↵b Average differences between alleles in the same strain as observed for all strains analyzed in this study. It is assumed that all strains were diploid and that a strain was homozygous if only one allele was observed.
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↵c A matrix was generated, describing pairwise differences between all possible combinations of the alleles observed in the strains. Each allele was used for comparisons as often as it occurred, so that the size of the matrix was equal to the square of 2 × no. of strains analyzed; thus the contribution of an individual allele to the average of differences was dependent on the frequency with which it was observed. It is assumed that all strains were diploid and that a strain was homozygous if only one allele was observed.
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↵d Two-sided t-test comparing observed differences and expected differences.
