Expression of ALS7. (A) RT–PCR amplification of VASES region of ALS7 mRNA from GPG cluster and noncluster C. albicans strains. Cells were grown in YEPD broth, and RT–PCR used primers MC5spF and MC5allF. Numbers on the left indicate DNA fragment size in kb. (B) Northern hybridization verifying full-size transcripts (7.2 kb and 7.8 kb) in Hun68 cells grown in YPD plus bovine serum. Varying amounts of mRNA were loaded, and blots were hybridized with a 2.1-kb probe generated from the HUN68 VASES region (the same bands were seen when the tandem repeat domain was used as a probe; data not shown), stripped, and reprobed with a 1.1-kb actin probe. Exposure time was 60 min.
