Characterization of ALS7 alleles by PCR and restriction analysis in the 42 strains of model Set 1. GPG cluster strains are marked by a shaded bar under the strain name, other strains by a hollow bar. (A) PCR amplification of the tandem repeat domain, using primers MC5inpf and MC5repr. (B) BsmAI digest of the products; a single BsmAI site in each 108-bp repeat is predicted from the nucleotide sequence, and so digestion of the PCR product should produce a 108-bp band plus three other bands of very similar molecular weight: an 80-bp band from the 5′ flanking sequence and bands of 95 bp and 116 bp from the 3′ flanking sequence. In addition, there should be a 270-bp band from the 3′ flanking sequence. (C) Amplification of the VASES region, using primers MC5spF andMC5pf. Numbers at the right of the figure give fragment sizes in kb.
