Expanding the Use of Zymography by the Chemical Linkage of Small, Defined Substrates to the Gel Matrix

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Figure 4
Figure 4

Silver and activity staining of E. coli proteins separated by 2D electrophoresis. The left half of this figure is a silver-stained gel that shows the position of polypeptides following 2D SDS PAGE. The right half is an autoradiography of the same gel that shows the results of an in-gel assay using as substrate the Acr#1 oligonucleotide radiolabeled at the 5′ end. An arrow in each half of the figure indicates the position of spots corresponding to nuclease digestion of the substrates.

This Article

  1. Genome Res. 13: 1961-1965

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