Table 6.
Effect of Using Multiple Reading Frames in PMSG Analysis to Detect All Possible Single Nucleotide Substitutions in Exons 2 and 3 of the RDS/Peripherin Gene
|
|
12-Da detection threshold |
5-Da detection threshold |
|||||
|---|---|---|---|---|---|---|---|
|
|
Total |
Nonsynonymous |
Total |
Nonsynonymous |
|||
| A. Predicted number of substitutions not detected in 252-nt exon 2 sequence (756 possibilities) | |||||||
| RF1 | 231 (30.5%) | 65 (8.6%) | 214 (28.3%) | 52 (6.9%) | |||
| RF1, RF4 | 151 (20.0%) | 54 (7.1%) | 141 (18.6%) | 39 (5.2%) | |||
| RF1, RF2 | 88 (11.6%) | 42 (5.6%) | 80 (10.5%) | 34 (4.5%) | |||
| RF1, RF2, RF4 | 52 (6.9%) | 31 (4.1%) | 44 (5.8%) | 26 (3.4%) | |||
| B. Predicted number of substitutions not detected in 213-nt exon 3 sequence (639 possibilities) | |||||||
| RF1 | 225 (35.2%) | 76 (11.9%) | 213 (33.3%) | 67 (10.5%) | |||
| RF1, RF3 | 152 (23.8%) | 55 (8.6%) | 142 (22.2%) | 44 (6.9%) | |||
| RF1, RF6 | 31 (4.9%) | 21 (3.3%) | 19 (3.0%) | 12 (1.9%) | |||
| RF1, RF3, RF6
|
18 (2.8%)
|
16 (2.5%)
|
9 (1.4%)
|
7 (1.1%)
|
|||
