Detection and Assignment of Mutations and Minihaplotypes in Human DNA Using Peptide Mass Signature Genotyping (PMSG): Application to the Human RDS/Peripherin Gene

Table 2.

PMSG Mass Predictions Generated From the DNA Sequencing Results Showing Two Possible Bases at Four Positions in the DNA Being Analyzed


Base present at SNP locations

Predicted masses

1147
1166
1250
1291
Forward
Reverse
Allele
G A G C 12311.5 12137.3 I
G A G T 12311.5 12209.3 IV
G A A C 12369.5 12127.2
G A A T 12369.5 12199.3
G G G C 12339.5 12103.2
G G G T 12339.5 12175.3
G G A C 12397.5 12093.2
G G A T 12397.5 12165.3
C A G C 12310.5 12236.4
C A G T 12310.5 12308.5
C A A C 12368.5 12226.4 II
C A A T 12368.5 12298.4
C G G C 12338.5 12202.4
C G G T 12338.5 12274.4
C G A C 12396.5 12192.3 III
C
G
A
T
12396.5
12264.4

  • The masses are shown for the natural forward reading frame and the reverse reading frame used for PMSG analysis of exon 3. The final column shows the alleles that were identified in this study

This Article

  1. Genome Res. 13: 1944-1951

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