Comparative chromosome-painting signals from human and placental mammal painting probes labeled with biotin (green signals detected with Avidin-FITC) and digoxigenin (red signals detected Rhodamine labeled anti-digoxigenin antibodies). FISH signals of the human Hsa1 probe onto metaphase spreads of the Sei whale (a) and the two-toed sloth (b) hybridizing to the euchromatin of one chromosome pair in both species. (c) Ring-tailed lemur probes 3 (red) and 19 (green) delineate neighboring signals on squirrel chromosome 5 (arrows). (d) Hybridization results of flow-sorted chromosome probes from cat, lemur, elephant, horse, squirrel, and pig onto human chromosome 1. The chromosomal origin of each probe is labeled below each hybridization result. In the case of the lemur and elephant, the label refers to the flow-sorted peaks (e.g., p6 = peak six) from which the probes were made.
