Spotted Long Oligonucleotide Arrays for Human Gene Expression Analysis

Table 1.

Probe Characteristics


Probe group

Compositiona

Distinct GenBank identifiersb

Distinct UniGene clustersc

Not associated with a UniGene clusterc
Operon Human Genome Oligo Set Version 1 13,971 long oligonucleotide probesd 13,652 11,158 2120
Operon Human Genome Oligo Set Version 2 21,329 long oligonucleotide probesd 21,306 19,888 728
Affymetrix U95Av2 GeneChip
12,558 sets of oligonucleotides, ∼16 perfect match 25-mers per set
11,282
8328
1695
  • a Not including control probes

  • b According to GenBank accession numbers provided by the manufacturers

  • c UniGene Build 155 was used to map GenBank identifiers to gene clusters

  • d The large majority of oligonucleotides were 70-mers (Version 1) or 69-mers (Version 2). In a small number of cases, shorter probes were produced to keep predicted melting temperatures within the desired range (see Methods)

This Article

  1. Genome Res. 13: 1775-1785

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