Identification of Promoter Regions in the Human Genome by Using a Retroviral Plasmid Library-Based Functional Reporter Gene Assay

Table 1.

Sequence Analysis of 858 GFP- Positive Clones


Category

GFP+ population

# of GFP+ clones/total

% of GFP+ clones

# of GFP+ clones that also hit a CpG island

# of GFP+ clones that also hit exon 1 or intron 1 of a Refgene
A
    Within 500 bp upstream of a Refgene transcription start site GFP+ low 15/418 4% 9/15
GFP+ high 57/440 13% 49/57
    500bp-2kb upstream of a Refgene transcription start site GFP+ low 8/418 2% 0/8
GFP+ high 7/440 2% 3/7
B
    Within 2 kb upstream of transcription start site of predicted genes in >2 annotation tables GFP+ low 37/418 9% 13/37 19/37
GFP+ high 35/440 8% 23/35 17/35
C
    CpG islands only GFP+ low 34/418 8% 34/34 9/34

GFP+ high
51/440
12%
51/51
15/51
  • Redgene refers to known genes derived from RefSeq mRNA alignments

    Predicted gene annotation tables are from Genscan, Ensembl, Acembly, and Softberry

This Article

  1. Genome Res. 13: 1765-1774

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