Identification of Promoter Regions in the Human Genome by Using a Retroviral Plasmid Library-Based Functional Reporter Gene Assay

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Figure 3
Figure 3

Distribution of GFP-positive clones that are functionally validated in transient transfection reporter assays. Clones are divided into three groups based on the fractional increase in activity of the reporter gene. Category A: within 2 kb upstream of a Refgene transcription start site, 86% luc+/β-gal+. Category B: within 2 kb upstream of predicted genes in two or more annotation tables, 56% luc+/β-gal+. Category C: overlap with a CpG island, 73% luc+/β-gal+. Other: clones not in Categories A–C, 61% luc+/β-gal+.

This Article

  1. Published in Advance June 12, 2003, doi: 10.1101/gr.529803 Genome Res. 13: 1765-1774

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