Figure 2
FACS of the genomic DNA library into low and high fractions. (A) Histogram for retroviral plasmid pSKF-6 with no promoter and the positive control pSKF-6 with the human PGK promoter (right peak). (B) Histogram for retroviral plasmid pSKF-6 with no promoter and the 300–600-bp fragment library. Histograms represent cell number as a function of GFP fluorescence. GFP expression was measured by using flow cytometry with excitation at 488 nm and emission at 534 nm read as fluorescein.
