Qinghong Yang; Alla Lishanski; Wendy Yang; Sandra Hatcher; Henrietta Seet; Jeffrey P. Gregg

Allele-Specific Holliday Junction Formation: A New Mechanism of Allelic Discrimination for SNP Scoring

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Figure 4

General rules of primer design for the SNP genotyping assays based on allele-specific Holliday structure formation. (A) The primer designs for those GC-rich SNPs that do not require the addition of a GC-clamp. (B,C) Two alternative ways to design primers for the SNPs that require the addition of a GC-clamp.