Identification of Novel ErbB3-Interacting Factors Using the Split-Ubiquitin Membrane Yeast Two-Hybrid System

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Figure 4
Figure 4

ErbB3 and RGS4 form a complex in human cells. Cell lysates of HEK293T cells coexpressing ErbB3 and RGS4 were immunoprecipitated with either IgG antibody (lanes 2 and 3) or goat polyclonal anti-RGS4 antibody (lanes 4 and 5) using either 100 mM (lanes 2 and 4) or 150 mM NaCl (lanes 3 and 5), and analyzed by immunoblotting with either rabbit polyclonal anti-RGS4 antibody (upper panel) or mouse monoclonal anti-ErbB3 antibody (lower panel). One-tenth of the same extract was used as the input control (lane 1).

This Article

  1. Genome Res. 13: 1744-1753

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