
Hybridization of BAC/PAC DNA to salt-extracted invdup(20p) metaphase chromosomes. FISH signals from BAC/PACs were detected using FITC (green) and chromosomes counterstained with DAPI (blue). (A–E) BAC/PAC clones dj839B4, dJ416N4, bA103J8, dj1098F8, and dj79618, respectively. (Left, middle, right) Combined color image, black and white image for DNA staining, and FISH signals, respectively. The neocentromere position is indicated by an open arrow, whereas closed arrow points to the corresponding normal 20p12 region. Scale bar, 1μm.











