Selectively Expressed Genes With Uncharacterized Subcellular Localization From the SAGE Results
|
Fold |
Tag sequence |
Count |
3′RACE product |
UniGene ID |
||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Transcripts on the microarray selectively expressed in activated Th1-polarized lymphocytes | ||||||||||
| Th1/Th2 | Th1 | Th2 | ||||||||
| 10.0 | CAGGTGCTGT | 10 | 1 | hypothetical protein | Hs.92374 | |||||
| 9.0 | GAATTCCTCG | 9 | 0 | hypothetical protein | Hs.301732 | |||||
| 6.0 | TCTGCAATGA | 6 | 0 | hypothetical protein | Hs.8170 | |||||
| 4.0 | AATCCCGCAA | 4 | 0 | hypothetical protein | Hs.268561 | |||||
| 4.0 | AGTTTGGGCT | 4 | 0 | hypothetical protein | Hs.9911 | |||||
| 4.0 | CCTTTGAACA | 4 | 0 | multiple match | Hs.321130 | |||||
| 4.0 | GCCTCCTGTC | 4 | 0 | chromosome 16 ORF 5 | Hs.7765 | |||||
| 4.0 | GTTTTCCGGT | 4 | 0 | CGI-94 protein | Hs.111449 | |||||
| 4.0 | GTGTAAATGG | 4 | 0 | CGI-101 protein | Hs.286131 | |||||
| 4.0 | CTGCCATAAC | 4 | 0 | CGI-97 protein | Hs.110445 | |||||
| Transcripts on the microarray selectively expressed in activated Th2-polarized lymphocytes | ||||||||||
| Th2/Th1 | Th1 | Th2 | ||||||||
| 5.0 | GCCACACCCC | 0 | 5 | hypothetical protein | Hs.7718 | |||||
| Transcripts on the microarray increased in activated T lymphocytes | ||||||||||
| Activated T/resting T | activated T | resting T | ||||||||
| 95 | GGCTGGTCTG | 95 | 0 | hypothetical protein | Hs.337986 | |||||
| 34 | TACTGGTGGA | 34 | 0 | hypothetical protein | Hs.136912 | |||||
| 9 | GAATTCCTCG | 9 | 0 | hypothetical protein | Hs.301732 | |||||
| 9 | CTTGTAAACT | 9 | 0 | hypothetical protein | Hs.57209 | |||||
| 9 | GCCTCACTGC | 9 | 0 | clone IMAGE:3881549, mRNA | Hs.346735 | |||||
| 8 | CTGATGGCAG | 8 | 0 | hypothetical protein | Hs.334787 | |||||
| 8 | TTCTTGTTTG | 8 | 0 | Homo sapiens cDNA | Hs.250824 | |||||
| 7 | GCTACAGGTA | 7 | 0 | Homo sapiens cDNA | Hs.349306 | |||||
| 7 | ACTGTCCCCC | 7 | 0 | EST | Hs.50471 | |||||
| 7 | ATAATACTAA | 7 | 0 | EST | Hs.130058 | |||||
| 7 | TGATTATTGT | 7 | 0 | EST | Hs.182285 | |||||
| 7 | TCAGAGAATA | 7 | 0 | hypothetical protein | Hs.99486 | |||||
| 6 | GACAGATGGA | 6 | 0 | KIAA1533 protein | Hs.83575 | |||||
| 6 | TGGGCCCGTG | 6 | 0 | clone MGC:16395 IMAGE:3939387, mRNA | Hs.11607 | |||||
| 6 | GCCAGGGGCC | 6 | 0 | KIAA1273 protein | Hs.23413 | |||||
| 5 | TAAGTTCCTT | 5 | 0 | hypothetical protein | Hs.237971 | |||||
| 5 | GAATTAAGCG | 5 | 0 | EST | Hs.245798 | |||||
| 5 | GCAGCCCGCG | 5 | 0 | hypothetical protein | Hs.71472 | |||||
| 5 | GGGGCAGCCC | 5 | 0 | hypothetical protein | Hs.65234 | |||||
|
5
|
GACAAGCCCA
|
5
|
0
|
hypothetical protein
|
Hs.180040
|
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The microarray includes the listed genes. Expression of each gene was confirmed by 3′RACE. 3′RACE products corresponded with the most probable GenBank matches
The tag sequence represents the 10-bp SAGE tag. Count indicates the number of times the tag was identified. Each tag number was normalized by using SAGE software by calculating 32,000
Fold change in expression was calculated as follows. After normalization of the number of each tag, the relative expression of each transcript was determined by dividing the number of tags observed in activated Th1, activated Th2, or resting T lymphocytes as indicated. To avoid division by zero, we used a tag value of one for any tag that was not detectable
