(A) Autoradiogram demonstrating the susceptibility of Hs.7718 gene product to proteinase K when translated in vitro in the presence of microsomal vesicles (microsomes). Equal aliquots of each translation reaction were exposed to proteinase K in the absence (-) or presence (+) of Triton X-100. (B) HEK293 cells overexpressing green fluorescent protein (GFP)–fused Hs.182285 gene product. HEK293 cells expressing GFP-fused Hs.182285 gene product (left) were fixed, and the membrane is labeled with DilC12 (center). Images were merged (right). When GFP-fused Hs.182285 gene product distribution is compared with the membrane labeling in red performed with DilC12, the GFP fluorescent signal was identical to the plasma membrane.
