Predicted structures of six hypothetical proteins and RT-PCR analysis of their expression level. By using an algorithm such as SOSUI and PSORTII, transmembrane domains of the six hypothetical proteins were predicted on the basis of the presumed human ortholog. Sequences were obtained from the public database. In the column predicted structure, the black oval represents putative signal sequence; the black boxes, putative transmembrane domains. RT-PCR was performed on total RNA isolated to confirm SAGE results. Th1, Th2, act T, and res T indicate activated Th1 lymphocytes, activated Th2 lymphocytes, activated T lymphocytes, and resting T lymphocytes, respectively. The Cy5/Cy3 fluorescent ratio is a value that is given to each gene by the microarray analysis; candidates of secretory proteins have Cy5/Cy3 fluorescent ratios >1.35.
