Analysis of mRNA With Microsomal Fractionation Using a SAGE-Based DNA Microarray System Facilitates Identification of the Genes Encoding Secretory Proteins

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Figure 2
Figure 2

Distribution analysis of RNA encoding proteins with characterized subcellular localization. (A) RT-PCR was performed on total RNA isolated from the free ribosomal and the microsomal fraction. GATA3 and G3PDH were selected for cytoplasmic proteins; CD48, CXCR4, and CCR7 for receptors; and IFNγ and MIP1β for secreted proteins. F and M indicate the free ribosomal and the microsomal fraction, respectively. (B) Microarray analysis gave a Cy5/Cy3 ratio to each gene. The free column shows the box plot of Cy5/Cy3 ratios of 150 genes encoding cytoplasmic proteins; the membrane column shows the box plot of Cy5/Cy3 ratios of 229 genes encoding secretory proteins. Each box includes 60% of genes encoding the cytoplasmic or secretory proteins.

This Article

  1. Published in Advance June 12, 2003, doi: 10.1101/gr.709603 Genome Res. 13: 1728-1736

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