Reproducibility and sensitivity of the ImmunoQuantArray. Raw Ct values for each of the 96 genes in the IQA are plotted. The linear regression best-fit line is shown and its correlation coefficient indicated. (A) Spleen cDNAs (left panel) or blood cDNAs (middle panel) from two C57BL/6J males. The average of the two spleen cDNA Cts is compared with the average two blood cDNA Cts (right panel). (B) The average spleen cDNA Cts of five BALB/cJ (left), three 129X1/SvJ (middle), or five BXSB/MpJ-Yaa+ (right) males was compared with the average spleen cDNA Cts of five C57BL/6J males. The point lying closest to the ordinate is 18S rRNA.
