Systematic Cloning of Treponema pallidum Open Reading Frames for Protein Expression and Antigen Discovery

Table 2.

Identity and Statistical Analysis of the 15 Treponema pallidum Genes Yielding the Highest Signal in a Chemiluminescent Immunoassay


Gene

Name

Sample number

Predicted protein size (kD)

Meana

St. dev

P value
TP0100 thioredoxin-like protein 20 c 3709.2 294 <0.001
TP0163 ABC transporter (TroA, Tromp1) 32 c 300.7 36 <0.001
TP0257 glycerophosphoryldiester phosphodiesterase (GlpQ) 46 c 1447.7 94 <0.001
TP0298 exported protein TpN38 51 c 277.7 16.5 <0.001
TP0319 membrane lipoprotein (TmpC) 59 c 11259.1 452 <0.001
TP0326 outer membrane protein 61 c 5017.7 550 <0.001
TP0435 lipoprotein, 17 kD (Tpp17) 79 c 17765.6 2583 <0.001
TP0088 conserved hypothetical protein 18 23 572.4 49 <0.001
TP0133 hypothetical protein 24 43 1769.3 91 <0.001
TP0183 hypothetical protein 38 31 8338.6 705 <0.001
TP0307 conserved hypothetical protein 54 37 880.9 105 <0.001
TP0327b hypothetical protein 62 20 214.1 58 0.198
TP0456b hypothetical protein 80 47 265.7 102 0.023
TP0469 conserved hypothetical protein 85 54 444.4 69 <0.001
TP0470b
conserved hypothetical protein
86
43
294.7
101
0.0034
  • a Mean value units correspond to relative light units detected

  • b Gene identified as statistically insignificant

  • c Predicted protein size was not included for previously characterized genes

This Article

  1. Genome Res. 13: 1665-1674

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