Finding exon-skipping events that are conserved between human and mouse: 3583 exon-skipping events were found in the human genome, using the methods described in Sorek et al. (2002). (A) For 980 of these human exons, a mouse EST spanning the intron that represents the exon-skipping variant was found. Human ESTs appear in purple; mouse ESTs are in light blue. (B,C) The two possible ways to identify an exon as conserved in mouse. (B) Identification of mouse ESTs that contain the exon, as well as the two flanking exons. (C) If the exon was not represented in mouse ESTs, the sequence of the human exon was searched against the intron spanned by the skipping mouse EST on the mouse genome. If a significant conservation (above 80%) was found, and the alignment spanned the full length of the human exon, the exon was declared as conserved.
