CDS Annotation in Full-Length cDNA Sequence

Table 2.

Annotated and Translated CDS by CDS Programs




Annotated

Translated

rsCDS

NCBI CDS

ProCrest

DECODER

Longest-ORF

Truncated-ORF

Manual
In-frame (complete) 10,759 10,759 5,287 8,343 9,477 9,517 10,409 0 43
In-frame (partial) 1,804 1,804 858 959 750 1,000 0 1,498 6
FS/stop (complete) 1,695 1,508 1,094 3 314 847 0 0 41
FS/stop (partial) 324 274 244 0 1 59 0 0 11
Total (clones) 14,582 14,345 7,483 9,305 10,542 11,423 10,409 1,498 101
Sensitivity (%)


52.2
64.9
73.5
79.6
72.6
10.4
0.7
  • The number of representative clones annotated and translated as complete or partial CDS is shown in the left two columns. “In-frame” means that the CDS region was determined without frameshift and/or stop codon error correction, and “FS/stop” means that the CDS region was completed after compensation for frameshifts or stop codons. The number of predicted CDS for each program, and its sensitivity, are shown in the following six columns. The sum of translated CDS regions calculated by each program was not 14,345 because some clones were translated by more than one program. 101 CDSs were not predicted by any program, but annotated manually

This Article

  1. Genome Res. 13: 1478-1487

Preprint Server