Targeting a Complex Transcriptome: The Construction of the Mouse Full-Length cDNA Encyclopedia

Table 4.

Estimation of Transcriptional Starting Points Identified by 5′ EST Mapping




5′ ESTs extending the CDSs

Clusters matching same genes

Singletons that extend CDSs
TSP <100 nt longer on genome 17,073 2350 42
New TSP >100 bp longer on genome 1669 516 29
Single tissue type (1) 4488 609 1269
Multiple tissue type (1) 224,934 7902 n.a.
Single developmental stage (1) (2) 16,418 1781 1269
Multiple developmental stage (1) (2) 213,509 6747 n.a.
Single library ID 2,078 269 1269
Multiple Library ID 227,849 8259 n.a.
Total, >10 bp (longer) 121,444 4934/8637 635
Total, ±10 bp 66,192 5115/8637 224
Total, <10 bp (shorter)
59,449
7030/8637
367
  • Only matches that extend existing CDS were considered

    Reference mRNAs with complete CDS without hit are not shown

    Clusters were prepared without using genome alignment and later aligned to the genome sequence

  • (1) Without taking into account library IDs 21, 23 and unrecognized mixed libraries

  • (2) Divided as preimplantation embryo, embryo after day 6, and adult

This Article

  1. Genome Res. 13: 1273-1289

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