Assessment of SAGE in Transcript Identification

Table 1.

Tag Mapping Accuracy Using Conceptual Transcripts Compared With Other Available Sequences


Test tags assigned to genes by mapping to...

Tags mapped unambiguously

Unambiguously mapped tags correctly assigned to genes
Full-length cDNAs (5606) 99% 100%
EST sequences (204,380) 94% 70%
Predicted protein coding sequences from genomic annotation (13,489) 97% 48%
Conceptual transcripts constructed from predicted protein coding sequences from genomic annotation, UTRs estimated (13,489) 96% 81%
Conceptual transcripts constructed from predicted protein coding sequences from genomic annotation, UTRs derived from EST data or estimated if ESTs unavailable (13,489) 96% 85%
Conceptual transcripts constructed from predicted protein coding sequences from genomic annotation, UTRs derived from EST and cDNA data or estimated if ESTs and cDNAs unavailable (13,489)
96%
93%

This Article

  1. Genome Res. 13: 1203-1215

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