Table 3.
Primers and Tissues used for RTPCR of Zinc-Finger Genes
|
Gene |
cDNA sourcea |
Primers |
Primer sequences |
|---|---|---|---|
| ZNF283 | Human brain Marathon-ready cDNA | Forward | CAGGAGGAGTGGGAATGCC, ATTCAGGGATGTGGCCATCGA |
| Reverse | CACATTGAGTTGATAGGCACTTAAATAATTCTT, AATTCTCTCATGTTTAACAAGGCTT | ||
| ZNF404 | Human full-length multitissue cDNA | Forward | GGAGTGGGAATATTTAAACTCGGAT |
| Reverse | TAAAGCCCTTCTTATATTCCTTACACTC | ||
| ZNF284 | Human testis Marathon-ready cDNA | Forward | TGGGGCTGCTGGACGTTT |
| Reverse | AACATACATTCCTGATCTACGGCTGAAA | ||
| ZNF234 | Human spleen Marathon-ready cDNA | Forward | CATGATGTATTCCTTTTAGAAAA |
| Reverse | CCTTGTAGAACTTCCTCCCTCT, CAAAGTTGAAGACATCAGT | ||
| ZNF227 | Human testis Marathon-ready cDNA | Forward | CTCCAGGGAGGAACTGCGA |
| Reverse | GCAACTGTCGCATCTATAAGACTT | ||
| ZNF233 | Human testis Marathon-ready cDNA | Forward | GCTGTCAGTGGGCTATCAACCCTTCAAACT |
| Reverse | CATCTTCAGAGACCTGACTAGATTCTCCTG | ||
| ZNF285 | Human testis Marathon-ready cDNA | Forward | GAGCTGGCACTATTGGATAAA |
| Reverse | TTAAGTGTAGTTGCTGGTGAAGTT, GTGAAGGGAAGAGCTGC | ||
| ZNF229 | Human brain Marathon-ready cDNA | Forward | TCATGTCTCAGGAGCCATTGAG |
| Reverse | GATGAATAAGAAGAACCGATTTATACCT, CAAGACTCTTAACAGATTTCTCTCCTGT, CATCTTCTGAGAACTGGAAGTC | ||
| LOC147711 | Human brain Marathon-ready cDNA | Reverse | CAACTGTTGATTTCATACCAAA, GAGTGTGAACTCAGATGAGT |
| Forward | GAGCTGGCACTATTGGATAAA | ||
| Zfp114 | Mouse 15-Day Embryo marathon-ready cDNA | Forward | CCGTGGTCTTCAGCGAGGAGG |
| Reverse | CTGTCGCAGTGGGAGGGCTTCTTG | ||
| Zfp180 | Mouse 15-Day Embryo marathon-ready cDNA | Forward | GTCCTGACCAGTTGAGAGTC, GCACAGACTTCAAGATTGTAA |
|
|
|
Reverse
|
GCATTCTTCTCACACTGTCAC
|
-
↵a All cDNA preparations obtained from B.D. Clontech Corp; mouse samples from Swiss Webster outbred mouse tissues.
