Expression of anx11 genes. Dorsal (A) and lateral (B) views of notochord anx11a expression in a four-somite embryo. (C, D) Dorsal views of 15-somite embryos illustrating a shift in anx11a expression from the axial mesoderm to the adaxial mesoderm that is also observed in embryos that lack differentiated notochord (D; no tail-/-). (E) By 24 hpf, anx11a expression was observed in the paraxial mesoderm (arrowhead) and anus (arrow). (F) Lateral views of 24-hpf embryos reveal expression of both anx11a (top) and anx11b (bottom) in the periderm, arrowhead marks the anus. (G, H) Lateral views of 80-hpf larvae indicating greater levels of anx11a transcripts (G) in the liver (arrowhead) than that of anx11b (H). Embryonic and adult expression of anx11a determined by Northern analysis. (I) Total RNA was isolated from zebrafish embryos at selected stages from the one-cell stage to adult. Bar, 100 μm.
