Expression of anx5. (A) Lateral view of a 21-somite embryo revealing anx5 transcripts in blood island (arrowhead). Coronal view of a 26-somite embryo indicates anx5 expression in the most anterior domain (B) and in the olfactory placodes (C). (D) Ventral view of a 24-hpf embryo reveals intense anx5 expression in the olfactory placodes, hatching gland cells, and anus. (E) Sagittal section through an 80-hpf larvae reveals expression in the developing nasal epithelium, anus, and swim bladder. (F) Embryonic and adult expression of anx5 determined by Northern analysis. Total RNA was isolated from zebrafish embryos at selected stages from the one-cell stage to adult. (A—C) Bar, 100 μm; (D, E) bar, 200 μm.
