Selecting Open Reading Frames From DNA

Table 1.

Display Efficiency With and Without β-Lactamase

Construct ELISA signals
Lysozyme BSA
pDAN5 D1.3 0.934 ± 0.120 0.090 ± 0.021
pPAO1 D1.3 before cre 0.126 ± 0.013 0.086 ± 0.007
pPAO1 D1.3 after cre 0.878 ± 0.080 0.102 ± 0.038
  • ELISA signals generated for phage containing the D1.3 scFv cloned into a standard phage display vector (pDAN5), pPAO2 with β-lactamase still present (before cre), and after β-lactamase has been removed.

This Article

  1. Genome Res. 13: 980-990

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