Figure 6.
Identification of kanamycin clones. Three kanamycin-specific primers were used in two separate PCR reactions (see Methods for details) with different numbers of estimated clones from the library indicated. Amplification is noted when 100 templates are present, indicating that the kanamycin sequence is present at an approximate level of 1/100. The 15–13 primers amplify an ORF of no biological significance that is not represented in the library. The bars on the left indicate the length of the amplification products.
