Figure 5.
Sequence analysis of ampicillin-selected open reading frame clones. The pET28b-tTG plasmid is represented linearly, with all ORFs >50 amino acids (150 bp) indicated. In addition, ORFs corresponding to kanamycin, tTG, lacI, and rop are hatched. Regions of the plasmid identified after sequencing 43 clones obtained after selection on ampicillin and removal of the β-lactamase after passage through a Cre-expressing strain are shown as black lines beneath the appropriate frame.
