Selecting Open Reading Frames From DNA

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Figure 2.Figure 2.
Figure 2.

pPAO2, plasmid map, and polylinker sequence. (A) The plasmid map of pPAO2. (B) The polylinker sequence between theHindIII and EcoRI sites of pUC119. The amino acid sequence of the in-frame construct is given above the DNA sequence. FS indicates a frameshift, which can only be overcome if an in-frame DNA fragment is cloned via the LIC approach. (C) The LIC cloning procedure in detail.

This Article

  1. Genome Res. 13: 980-990

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