Figure 1.
The scheme for open reading frame selection. The scheme for selecting DNA fragments encoding ORFs. Random fragments are cloned upstream of a β-lactamase gene. Those fragments that are ORFs permit readthrough into the β-lactamase gene and confer ampicillin resistance. Those that are out of frame, or contain stop codons, do not survive. After selection on ampicillin, the β-lactamase gene can be removed by passage through bacteria expressing Cre recombinase. The selected ORF can then be displayed on phage.
