Unbiased Whole-Genome Amplification Directly From Clinical Samples

Table 3.

Genotyping of Nine STR Loci and Amelogenin Locus on MDA-Amplified Product From Whole Blood, Finger Stick Blood, and Buccal Swab

Sample # D3S1358 vWA FGA D8S1179 D21S11 D18S51 D5S818 D13S317 D7S820 AMEL Concordance with gDNA
1 2 1 2 1 2 1 2 1 2 1 2 1 2 1 2 1 2 1 2 WB FP BS
1 14 16 17 18 27 27 16 17 29 33.2 12 18 9 11 13 13 11 11 X Y 100% 100% 100%
2 14 16 16 19 20 22 12 15 28 31 15 16 11 12 9 12 9 10 X Y 100% 100% 100%
3 16 18 14 18 21 22 15 15 29 32 15 19 11 12 11 12 9 12 X Y 100% 100% 100%
4 16 19 14 14 24 25 13 16 29 32.2 14 14 11 12 8 8 10 11 X X 100% 100% 100%
5 15 15 17 18 21 21 15 15 29 29 14 21 12 13 8 12 10 12 X Y 100% 100% 100%
6 15 17 16 16 19 22 13 13 28 30 13 18 12 12 9 11 12 13 X Y 100% 100% 100%
7 15 17 16 17 20 21 13 14 29 30 12 15 10 11 12 14 10 12 X X 100% 100% 100%
8 16 17 16 17 23 23 15 17 29 31.2 11 14 10 12 12 13 11 11 X Y 100% 100% 100%
9 14 15 16 17 19 21 14 14 28 29 16 17 12 12 12 12 8 11 X X 100% 100% 100%
10 15 15 17 17 23 24 15 16 30 31.2 15 15 11 12 11 11 12 12 X X 100% 100% 100%
11 14 15 14 17 20 22 13 14 29 29 15 18 11 12 8 11 8 10 X X 100% 100% 100%
12 17 18 16 17 21 22 12 13 28 30 10 15 11 12 11 12 9 9 X Y 100% 100% 100%
13 16 17 16 17 22 24 14 15 29 33.2 12 17 11 11 12 13 9 11 X Y 100% 100% 100%
14 14 17 15 19 20 22 13 14 29 30 13 15 11 12 11 11 9 10 X Y 100% 100% 100%
15 15 17 14 17 24 26 10 13 30 32.2 12 13 11 12 9 10 9 13 X X 100% 100% 100%
16 17 17 15 16 22 24 13 14 30 30 14 17 10 11 10 11 8 10 X X 100% 100% 100%
17 16 17 14 18 21 22 12 15 30 30.2 11 14 11 12 9 10 8 10 X Y 100% 100% 100%
18 17 17 16 18 20 24 11 12 30 30.2 17 17 9 11 11 12 12 12 X Y 100% 100% 100%
19 15 16 17 18 23 23 15 15 31 32.2 14 15 10 12 11 13 10 12 X X 100% 100% 100%
20 15 17 14 17 19 20 14 15 30 31 12 15 12 13 10 11 11 11 X X 100% 100% 100%
  • The first column shows the sample ID number of the 20 individuals tested. The next 10 columns show the 10 loci tested for genotyping, which were D3S1358, D5S818, D7S820, D8S1179, D13S317, D18S51, D21S11, FGA, vWA, and Amelogenin. Each locus consists of two parental alleles (columns 1 and 2). The numbers in each box refer to the number of short tandem repeat (STR) sequences. For example, the genotype of individual number 1, locus D3S1358, is heterozygous for 14 and 16 STR repeats. The small table on the right shows the percentage genotype concordance with the genomic DNA control for the biological specimens tested. (WB) Whole blood; (FP) finger stick; (BS) buccal swab.

This Article

  1. Genome Res. 13: 954-964

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