Table 2.
Genotyping of Five SNP Loci on MDA-Amplified Product From Whole Blood, Finger Stick Blood, and Buccal SWAB
| Sample # | CYP2C19*2 | CYP2D6*8 | CYP2C19*3 | CYP2D6*6 | CYP2D6*4 | Concordance with gDNA | ||
| WB | FP | BS | ||||||
| 1 | GA | GG | GG | no deletion | GG | 100% | 100% | 100% |
| 2 | GG | GG | GG | no deletion | GG | 100% | 100% | 100% |
| 3 | GG | GG | GG | no deletion | GG | 100% | 100% | 100% |
| 4 | GA | GG | GG | no deletion | GG | 100% | 100% | 100% |
| 5 | GA | GG | GG | no deletion | GG | 100% | 100% | 100% |
| 6 | AA | GG | GG | no deletion | GG | 100% | 100% | 100% |
| 7 | GA | GG | GG | no deletion | GG | 100% | 100% | 100% |
| 8 | GA | GG | GG | no deletion | GG | 100% | 100% | 100% |
| 9 | GA | GG | GG | no deletion | GG | 100% | 100% | 100% |
| 10 | GG | GG | GG | no deletion | GG | 100% | 100% | 100% |
| 11 | GG | GG | GG | no deletion | GG | 100% | 100% | 100% |
| 12 | GG | GG | GG | no deletion | GG | 100% | 100% | 100% |
| 13 | GG | GG | GG | no deletion | GA | 100% | 100% | 100% |
| 14 | GG | GG | GG | no deletion | GG | 100% | 100% | 100% |
| 15 | GG | GG | GG | no deletion | GG | 100% | 100% | 100% |
| 16 | GA | GG | GA | no deletion | GG | 100% | 100% | 100% |
| 17 | GG | GG | GG | no deletion | GA | 100% | 100% | 100% |
| 18 | GA | GG | GG | no deletion | GG | 100% | 100% | 100% |
| 19 | GG | GG | GG | no deletion | GG | 100% | 100% | 100% |
| 20 | GA | GG | GG | no deletion | GG | 100% | 100% | 100% |
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The first column shows the sample ID number of the 20 individuals tested. The next five columns show the five loci tested for genotyping, which were CYP2C19*2 (G/A), CYP2D6*8 (G/T), CYP2C19*3 (G/A), CYP2D6*6 (T deletion), and CYP2D6*4 (G/A), by TaqMan assay for allelic discrimination. The small table on the right shows the percentage genotype concordance with the genomic DNA control for the biological specimens tested. (WB) Whole blood; (FP) finger stick; (BS) buccal swab.
