DNA Analysis by Fluorescence Quenching Detection

Table 1.

Allele Frequency Estimates From Constructed Mixtures with Allele Frequency Ranging from 5% to 95%

Known allele frequency S2/S1 S.D. Estimated allele frequency S.D.
0.05 34.33 2.47 0.09 0.022
0.1 13.25 4.82 0.13 0.023
0.15 8.63 1.43 0.16 0.025
0.2 7.12 0.95 0.19 0.014
0.25 4.36 0.20 0.27 0.009
0.3 3.54 0.13 0.31 0.008
0.35 3.01 0.17 0.35 0.012
0.4 2.64 0.20 0.38 0.018
0.45 1.94 0.11 0.45 0.014
0.5 1.61 0.042 0.50 0.007
0.55 1.34 0.091 0.55 0.017
0.6 1.10 0.064 0.60 0.015
0.65 0.92 0.058 0.64 0.016
0.7 0.70 0.060 0.70 0.019
0.75 0.56 0.043 0.75 0.016
0.8 0.42 0.030 0.80 0.013
0.85 0.28 0.041 0.86 0.018
0.9 0.23 0.012 0.89 0.006
0.95 0.098 0.046 0.96 0.028
  • S2/S1, slope ratio; S.D., standard deviation.

This Article

  1. Genome Res. 13: 932-939

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