Tn7-Based Genome-Wide Random Insertional Mutagenesis of Candida glabrata

(Downloading may take up to 30 seconds. If the slide opens in your browser, select File -> Save As to save it.)

Click on image to view larger version.

Figure 4.
Figure 4.

Southern analysis of Candida glabrata transformants derived from two mutagenized fosmids. The probes are the URA3 gene from Tn7 UKR (lanes 1–9). All DNAs were digested with BcgI and BamHI prior to agarose gel electrophoresis. (Lane 1) C.g., genomic DNA from a pool of 35 Ura+ transformants derived from transformation with mutagenized fosmid 1. (Lane 2) E. coli, genomic DNA from 150 pooled Tn7 insertions in fosmid 1. (Lane3) U, DNA from unmutagenized fosmid 1. (Lane 4)C.g., genomic DNA from a pool of 44 Ura+transformants derived from transformation with mutagenized fosmid 2. (Lane 5) E. coli, genomic DNA from 150 pooled Tn7 insertions in fosmid 2. (Lane 6) C.g., genomic DNA from a pool of 5 Ura+ transformants derived from transformation with Tn7-mutagenized pBAC-Bcg3. (Lane7) DNA from unmutagenized fosmid 2. (Lane 8)E. coli, genomic DNA from >1000 pooled Tn7insertions in plasmid pBAC-Bcg3. (Lane 9) Cont, control pIC31 digested with PstI.

This Article

  1. Published in Advance April 14, 2003, doi: 10.1101/gr.848203 Genome Res. 13: 905-915

Preprint Server



Current Issue

  1. January 2026, 36 (1)
  1. Current Issue
  1. Alert me to new issues of Genome Research