Figure 3.
PCR amplification for 11 Class 3 transformants using three primer pairs. (A) Genomic primers flanking the Tn7 insertion site and oriented toward the insertion. (B) One genomic primer combined with a Tn7R primer. (C) Genomic primer combined with the Tn7L primer. Note that the three positive transformants for B and C are the only transformants positive for the longer PCR product in A corresponding to the Tn7-disrupted band. Control lanes: (wt) BG14 genomic DNA, (C) pIC14 DNA used as template for PCR.
