Tn7-Based Genome-Wide Random Insertional Mutagenesis of Candida glabrata

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Figure 1.
Figure 1.

Maps of mini-Tn7 derivatives used. Tn7 UKR (URA3 Kan · R6Kγ ori) is a 3.108-kb derivative of mini-Tn7 SpeI-KmR-NotI containing the conditional origin of replication R6Kγ and the Saccharomyces cerevisiae URA3 gene. Relevant restriction sites are shown. Tn7 UKR-H is a 5.118-kb element derived from Tn7 UKR that contains the Klebsiella pneumoniae hph gene (which confers resistance to hygromycin B) driven by the PGK1promoter from S. cerevisiae. The arrows underneath each map indicate the direction of transcription.

This Article

  1. Published in Advance April 14, 2003, doi: 10.1101/gr.848203 Genome Res. 13: 905-915

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