Figure 3.
Performance comparison of reduced-input labeling to standard protocol for use with 60-mer oligo microarrays. Decreasing amounts of total RNA from mouse E12.5 embryo and placenta were used to prepare labeled linear-amplified targets, using one round of amplification for 250- and 50-ng and two rounds for 10- and 2-ng inputs. Each spot represents averaged ratios from four dye-swapped microarrays, each containing eight replicates of each probe. The correlation coefficient, slope, number of significant transcripts, overlap with the reference set of 784 significant transcripts, specificity, and sensitivity are shown beneath each panel.
