Searching Gene Ontology Annotation (GOA) database with Sequence Retrieval System (SRS). (A) To find all annotated proteins that function as electron transfer flavoproteins and that have an experimental evidence code (Non-IEA), the “goid” field is searched for the GO identifier “0008246”in the GOA database. In the “combined searches with” section of the tool bar, the “BUTNOT” option is selected and ”IEA” (the GO evidence code for “inferred from electronic annotation”) is entered in the “evidence” field. (B) This produces a query result, which displays all proteins manually assigned the function of “electron transport flavoprotein” using published literature. Associations made by electronic inference are filtered out and results displayed in the “gene association file” format. (C, D) A further facility of SRS is its ability to link to databases that may or may not contain direct references to each other. As such, the last search can be extended to display EMBL/GenBank/DDBJ accession numbers by selecting the “link” option and choosing the EMBL database and “submit link.”
