Model 1 Predictions of Immune Genes Among Well Characterized Genes
| Gene name | Acc. no. | Evidence of NF-κB regulation | Immune gene | Model score | Functions in immune system |
| RANTES | AF088219 | Promoter study(1) | +(2) | 0.935 | Activator of variety of leukocytes |
| ELAM-1 | AL021940 | Promoter study(3) | +(4) | 0.833 | Migration of leukocytes |
| HLA-B | AJ250917 | Promoter study(5) | + | 0.935 | MHC class I molecule |
| IL8 | M26383 | Promoter study(3) | +(6) | 0.850 | Acute inflammation |
| GROγ | M36821 | Promoter study(3) | +(7) | 0.850 | Macrophage inflammatory protein |
| CD83 | NM_004233 | Promoter study(8) | +(9) | 0.833 | Dendritic cells maturation |
| NF-κB2 | S76638 | Promoter study(3) | +(3) | 0.739 | Regulator of immune response |
| MAJCAM1 | U43628 | Promoter study(3) | +(10) | 0.714 | Migration of leukocytes |
| IL6 | X04430 | Promoter study(3) | +(11) | 0.677 | Acute immune response |
| TNF-β | D12614 | Promoter study(3) | +(12) | 0.739 | Lymphoid organ development and chronic inflammation |
| CD69 | Z22576 | Promoter study(3) | +(13) | 0.871 | T-cell activation and function in other hematopoietic lineage cells |
| MAP3K8 | D14497 | Gene(14) | +(15) | 0.739 | Regulator of NF-κB in T cells; Involved in T-cell activation |
| MIP-3α | U64197 | Gene(16); array(17) | + | 0.714 | Macrophage inflammatory protein |
| IP3KC | D38169 | N/A | +(18) | 0.714 | T/B cell activation/differentiation; Signaling in monocyte/ macrophage |
| Nuclear matrix p84 | L36529 | N/A | − | 0.850 | N/A |
-
↵NF-κB binding site conserved inDrosophila, otherwise conserved in the mouse genome.
-
In the third column, experimental evidence on whether the promoters are regulated by NF-κB is found in the literature. “Promoter study” means the promoters have been cloned upstream of a reporter gene and expression assays demonstrated that the promoters are regulated by NF-κB. “Gene” means that a single gene expression assay of the candidate, usually by Northern blot, shows increased expression in cells after activating NF-κB with the stimulating factors. “Array” refers to microarray experiments showing that expression of the gene increases after NF-κB is activated in the cells. In the fourth column, a plus sign means the genes play a role in the immune system. The fifth column gives the score (π[x]) for the promoter in the LRA model. Ref. (1) Lee et al. 2000; Moriuchi et al. 1997. (2) Appay and Rowland-Jones 2001. (3) Baeuerle and Baichwal 1997. (4) Elangbam et al. 1997. (5) Girdlestone 2000; Gobin et al. 1998. (6) Harada et al. 1994. (7) Driscoll 1994. (8) McKinsey et al. 2000. (9) Robinson et al. 1998. (10) Elangbam et al. 1997. (11)Tilg et al. 1997; Akira and Kishimoto 1992. (12) Ruddle 1999. (13)Marzio et al. 1999; Ziegler et al. 1994. (14) Expression data in fibroblasts: OA, IL-1 (Chan et al. 1993); Spleen cells: Con A (Patriotis et al. 1993); T:αCD3, phorbol ester (Sanchez-Gongora et al. 2000). (15) Belich et al. 1999; Patriotis et al. 1993. (16) Expression data in THP-1:PMA; PBMC:LPS; I-HUVEC:TNFα (Hromas et al. 1997); Macrophage: Influenza A, Sendai virus (Matikainen et al. 2000). (17) Expression data in THP1:L. monocytogenes(Cohen et al. 2000); Macrophages:LDL (Shiffman et al. 2000). (18)Marshall et al. 2000; Chow et al. 1995; Ward and Cantrell 2001.
