Chromosomal Deletion Formation System Based on Tn5 Double Transposition: Use For Making Minimal Genomes and Essential Gene Analysis

Table 2.

The Summary of Analyzed Deletion/Plasmid Combinations for Different Insertions

Insert No. Δ Νο. Insert End Δ End Size Partial ORFs Complete ORFs Grow w/o IPTG
1 1.3 1073109 1094823 21714 b1028; b1012 b1013;putA; putP; b1016–1018; ycdB; phoH; b1021–1025;tra5Δ3; b1027  Y
2 2.8 2776930 2770104 6826 b2638; b2647 b2639–b2646  Y
3 3.1 4407731 4403605 4126 yjfl yjeB; vacB; yjfH  Y
4 4.2 3735047 3725227 9821 yiaB xylB; xylA; xylF; xylG; xylH; xylR; b3570  Y
4 4.6 3735047 3723521 11526 none yiaH; yiaA; yiaB; xylB; xylA; xylF; xylG; xylH; xylR;b3570  Y
4 4.9 3735047 3719490 15557 yi5b glyS; glyQ; yiaH; yiaA; yiaB; xylB; xylA; xylF; xylG;xylH; xylR;b3570  N
5 5.1 1224154 1210042 14112 mcrA; minD b1160-b1173; minE  Y
6 6.2 3045069 3042244 2825 bglA; gcvP ygfF  Y
7 7.1 2779269 2767042 12227 b2633; b2647 b2634–B2646  Y
8 8.4 3882592 3882500 92 none none  Y
9 9.7 4589240 4595093 5853 mdoB; tsr yjjN; yjjM; b4356  Y
10 10.3 4141018 4117696 23322 menA; frwC hsIU; hsIV; ftsN; cytR; priA; rpmE; yiiX; metJ; metB; metL; metF; katG; yijE; yijF; gldA; talC; ptsA; yijl  N
11 11.3 2793099 2795902 2803 gabP;b2668 ygaE; b2665–b2667  Y
12 12.17 14166 12088 2078 none dnaK  N
13 13.19 4542104 4546611 4507 fimC; fimH fimD; fimF; fimG  Y
14 14.17 3821524 3818998 2526 spoT rpoZ; gmk  N
15 15.17 1570686 1544492 26194 yddC; yddG fdnG; fdnH; fdnl; b1477; b1478; sfcA; rpsV;b1481; osmC; b1483–1491; xasA; gadB  Y

This Article

  1. Genome Res. 13: 644-653

Preprint Server