Chromosomal Deletion Formation System Based on Tn5 Double Transposition: Use For Making Minimal Genomes and Essential Gene Analysis

Table 1.

Genes Deleted in Strains Δ20-1 and Δ20-4

Deletion		B-Number	Map Position	Estimated Size(bp)
Δ20-1	Δ20-4	B-Number	Map Position	Estimated Size(bp)
1.1		b0116	2.8	1,424
1.2	4.1	b0288–b0315	6.5–7.2	29,606
1.3		b1188–b1197	26.6–26.9	11,667
1.4		b1335–b1452	30.1–32.8	124,647
	4.2	b1336–b1471	30.1–33.3	145,467
1.5	4.3	b1878–b1892	42.3–42.6	15,625
	4.4	b2379	53.8	1,238
1.6		b2649–b2655	60.0	3,604
	4.5	b2648–b2655	60.0	4,117
	4.6	b2853–b2861	64.5–64.6	4,293
	4.7	b3027	68.3	332
1.7		b3647	82.3	1,688
1.8	4.8	b3708–b3715	83.8–84.0	8,724
1.9		b4216	95.6	554
1.10	4.9	b4308–b4315	97.7–97.9	9,252
1.11		b4349–b4354	98.7–98.9	2,165

↵Deletion span based on b-number (Blattner et al. 1997).
↵Map position of genes lost within deletion span.
↵Size is estimated from left end of first deleted ORF to right end of last detected ORF.

This Article

Published in Advance March 12, 2003, doi: 10.1101/gr.611403 Genome Res. 2003. 13: 644-653

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